http://27.109.7.66:8080/xmlui/handle/123456789/522 It contains all document related to this collection Sat, 04 Apr 2026 17:29:52 GMT 2026-04-04T17:29:52Z http://27.109.7.66:8080/xmlui/handle/123456789/786 17β-estradiol modulates NGF and BDNF expression through ERβ mediated ERK signaling in cortical astrocytes Langhnoja, J M; L. K, Buch; Pillai, P P 17β-estradiol is known to exert neurotrophic and neuroprotective effects through classical estrogen receptors [ERs], ERα and ERβ, on a variety of cell types either by genomic or non-genomic actions. The actions of estradiol on glial cells are important to maintain metabolic functions of the nervous system. Astrocytes are considered to be active participants in brain activity because of their ability to release growth factors, including neurotrophins. Present in vitro studies show that 17β-estradiol modulates NGF and BDNF expression in time-dependent manner and ERK acts as secondary messenger for estradiol’s action. 17β-estradiol is involved in survival of cortical astrocytes. In conclusion, this study indicates vital role of ERβ mediated ERK signalling for regulation of NGF and BDNF expression along with cell viability of cortical astrocytes which further confirms the role of ERs, particularly ERβ in glial cells’ functions and viability. Biologia volume 73, 907–915 (2018) Wed, 01 Aug 2018 00:00:00 GMT http://27.109.7.66:8080/xmlui/handle/123456789/786 2018-08-01T00:00:00Z http://27.109.7.66:8080/xmlui/handle/123456789/785 Role of astrocytic MeCP2 in regulation of CNS myelination by affecting oligodendrocyte and neuronal physiology and axo–glial interactions Lipi, B; Jaldeep, L; Prakash, P Astrocytes perform several critical functions such as promoting neuronal maturation, neuronal survival, maintaining and supporting neurons and oligodendrocytes. Astrocytes participate in the formation of nodes of Ranvier. Recently, studies emphasizing on the role of astrocytes in regulating myelination by secreting pro-myelinating factors like growth factors, neurotrophins and ECM proteins, have been investigated by many researchers. Methyl-CpG-Binding Protein 2 (MeCP2), an epigenetic protein, binds to CpG islands in the genome and induces multiple gene regulatory functions by conforming changes in the chromatin structure and resulting in cell-specific gene expression. MeCP2 deficient astrocytes have been linked with abnormal neuronal function including decreased dendritic arborization and decreased dendritic outgrowth. However, role of astrocytic MeCP2 in central nervous system myelination is largely not known. The data from the current study indicate altered mRNA levels (Lif, Cntf, Pdgfa, Cxcl10) of astrocyte-secreted factors involved in myelination. Bdnf and Ngf mRNA levels were also altered in MeCP2 knockdown astrocytes. Moreover, the secreted BDNF levels were significantly altered whereas there were no significant changes in NGF secretion. We also observed that astrocytic MeCP2 affects the morphology, physiology and survival of oligodendrocytes and neurons—two of the key players in myelination. Further, we report that some of the axo–glial interaction genes, namely Caspr, Notch1, Nf155 and Nrg1 are under the regulation of astrocytic MeCP2 along with key myelin genes and proteins. Experimental brain research, 236, (7), 2018, pages3015–302 Wed, 01 Aug 2018 00:00:00 GMT http://27.109.7.66:8080/xmlui/handle/123456789/785 2018-08-01T00:00:00Z http://27.109.7.66:8080/xmlui/handle/123456789/780 The effect of different chemical treatments and salt stresson the germination potential of tavernieracuneifolia (roth) ali seeds P, Mangalorkar; Rana, K; Parikh, Rahul; Nagar, p Tavernieracuneifoliais an ethnobotanically important traditional medicinal plant of Semi-Arid region of India belonging to the family of Fabaceae. As the plant is ecologically identified as glycophyte the seeds were treated with various salts. Effect of various salts (KCl, MgCl2, NaCl, Na2CO3, MgSO4,) on the germination of T. cuneifolia seed was studied. Germination decreased with increase in salinity. The inhibition of germination by salt solution was in the order of NaCl> MgCl2> MgSO4>KCl. Non-germinated seeds under various salt treatments when transferred to distilled water recovered completely, indicating little ionic effect of salinity on seed germination and viability. Germination rate was highest in those seeds which were treated with concentrated sulphuric acid with the germination percentage to be 80 Journal of Chemical, Biological and Physical Sciences, 2014 4(1):369-374 Wed, 01 Jan 2014 00:00:00 GMT http://27.109.7.66:8080/xmlui/handle/123456789/780 2014-01-01T00:00:00Z http://27.109.7.66:8080/xmlui/handle/123456789/779 Design, Synthesis, In Silico Studies and In Vitro Anticancer Activity of 3-(4-Methoxyphenyl)azetidine Derivatives Parmar, D. R.; Rayani, R. H.; Vala, A. G.; Kusurkar, R.V.; Manvar, R.K.; Talukdar, S.N.; Preeti; Zunjar, V.; Battula, S.; Soni, J Y. A series of 3‐(4‐methoxyphenyl)azetidine analogues were synthesized and screened for their in vitro anticancer activity against nine different human cancer cell lines using the cell counting kit‐8 (CCK‐8) assay. The synthesized molecules were characterized by 1H NMR, 13C NMR, LCMS and IR analysis. The toxicity, bioavailability and lipophilicity of all the synthesized compounds were predicted by using osiris and molinspiration model. Molecular docking study revealed that, compound 6‐(3‐(3‐(2‐aminopyridin‐4‐yl)‐4‐methoxyphenyl)azetidin‐1‐yl)picolinonitrile (4 A‐17) and 6‐(3‐(4‐methoxy‐3‐(2‐methoxypyridin‐4‐yl)phenyl)azetidin‐1‐yl)picolinonitrile (4 A‐19) were found to be potential inhibitor of human topoisomerase IIα. The cell viability studies exhibited promising antiproliferative activities of the novel synthesized compounds. 4 A‐17 (EC50 0.03 μM) was found to be more potent than standard Doxorubicin (EC50 0.07 μM) in U251 cancer cell lines. Similarly, 4 A‐19 showed considerable potency against four different cancer cell lines (HepG2, U251, A431, 786‐O) with EC50 values ranging from 0.46 to 2.13 μM. These primary findings supported that molecule 4 A‐17 and 4 A‐19 should be subjected to further studies and lead optimization. Twenty two substituted 3‐(4‐methoxyphenyl)azetidines were synthesized, screened for anticancer activity against nine different cancer cell lines by CCK‐8 assay. In silico study revealed 6‐(3‐(3‐(2‐aminopyridin‐4‐yl)‐4‐methoxyphenyl)azetidin‐1‐yl)picolinonitrile (4 A‐17) and 6‐(3‐(4‐methoxy‐3‐(2‐methoxypyridin‐4‐yl)phenyl)azetidin‐1‐yl)picolinonitrile (4 A‐19) as potential human topoisomerase IIα inhibitor. In U251 cells, 4 A‐17 (EC50 0.03 μM) showed more potency than standard Doxorubicin (EC50 0.07 μM). 4 A‐19 found to be more potent than Doxorubicin in HepG2. The cellular toxicity study of the novel compounds showed selectivity on cancerous cells over normal cells (HEK‐293). Chemistry Select, 5(45):2020,PP.14296-14302 Tue, 01 Dec 2020 00:00:00 GMT http://27.109.7.66:8080/xmlui/handle/123456789/779 2020-12-01T00:00:00Z