Abstract:
Repeated and prolonged use of synthetic colours has thrown up many issues related to environment and human health. Hence, there is an increasing demand for “biocolour” from natural sources which can replace artificial synthetic colours. Natural colourants are generally obtained from plant, animal or micro organisms. Microbial pigments have some advantages over plant and animal species and so it is of much importance for pigment production. Leifsonia aquatica, Pseudomonas aeruginosa, Serratia marcescens were isolated for yellow, green and red colour pigment respectively, from natural environmental source and highest colour production was achieved at 25 ºC, 150 rpm in 72 h incubation time. Glycerol content was added for enhancement for good visibility of colour. Isolated pigment producing species were confirmed by fully automated Vitek 2 and Phoenix 100 system. Extracted pigments were analysed by TLC and various confirmation tests were carried out. Antimicrobial properties of extracted pigments were also evaluated. Extracted pigment can be applied on selected fabric at optimized conditions, using combination of mordants and may be checked/evaluated for washing, rubbing, light and temperature fastness. This indicates the ability of the pigment to be used as colourant for fabric and a good alternative source to replace synthetic dyes in textiles.
