NAVRACHANA UNIVERSITY

A thermosensitive low molecular weight hydrogel as scaffold for tissue engineering

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dc.contributor.author Ziane, Sophia
dc.contributor.author Schlaubitz, Silke
dc.contributor.author Miraux, Sylvain
dc.contributor.author Patwa, Amit
dc.contributor.author Lalande, Charlotte
dc.contributor.author Bilem, Ibrahim
dc.contributor.author Lepreux, Sébastien
dc.contributor.author Rousseau, Benoît
dc.contributor.author Le Meins, Jean-François
dc.contributor.author Latxague, Laurent
dc.contributor.author Barthélémy, Philippe
dc.contributor.author Chassande, Olivier
dc.date.accessioned 2019-06-14T05:26:11Z
dc.date.available 2019-06-14T05:26:11Z
dc.date.issued 2012
dc.identifier.issn 1473-2262
dc.identifier.uri http://27.109.7.66:8080/xmlui/handle/123456789/582
dc.description European Cells & Materials, Vol-23, 2012, p. 147-160 en_US
dc.description.abstract Hydrogels that are non-toxic, easy to use, cytocompatible, injectable and degradable are valuable biomaterials for tissue engineering and tissue repair. However, few compounds currently fulfi l these requirements. In this study, we describe the biological properties of a new type of thermosensitive hydrogel based on low-molecular weight glycosyl-nucleosyl-fl uorinated (GNF) compound. This gel forms within 25 min by self-assembly of monomers as temperature decreases. It degrades slowly in vitro and in vivo. It induces moderate chronic infl ammation and is progressively invaded by host cells and vessels, suggesting good integration to the host environment. Although human adult mesenchymal stem cells derived from adipose tissue (ASC) cannot adhere on the gel surface or within a 3D gel scaffold, cell aggregates grow and differentiate normally when entrapped in the GNF-based gel. Moreover, this hydrogel stimulates osteoblast differentiation of ASC in the absence of osteogenic factors. When implanted in mice, gel-entrapped cell aggregates survive for several weeks in contrast with gel-free spheroids. They are maintained in their original site of implantation where they interact with the host tissue and adhere on the extracellular matrix. They can differentiate in situ into alkaline phosphatase positive osteoblasts, which deposit a calcium phosphate-rich matrix. When injected into subcutaneous sites, gel-encapsulated cells show similar biological properties as implanted gel-cells complexes. These data point GNF-based gels as a novel class of hydrogels with original properties, in particular osteogenic potential, susceptible of providing new therapeutic solutions especially for bone tissue engineering applications. en_US
dc.language.iso en_US en_US
dc.publisher ECM Journal en_US
dc.subject Low molecular weight gel en_US
dc.subject Supramolecular assemblies en_US
dc.subject Nucleoside amphiphiles en_US
dc.subject Adipose tissue derived stem cells en_US
dc.subject Biocompatibility en_US
dc.title A thermosensitive low molecular weight hydrogel as scaffold for tissue engineering en_US
dc.type Article en_US


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