NAVRACHANA UNIVERSITY

Antithrombocytopenic activity of carpaine and alkaloidal extract of carica papaya linn. Leaves in busulfan induced thrombocytopenic wistar rats

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dc.contributor.author zunjar, Vishwanath
dc.contributor.author Das, Ranjit Prasad
dc.contributor.author Jivrajani, Mehul
dc.contributor.author Trivedi, Bhavna
dc.contributor.author Nivsarkar, Manish
dc.date.accessioned 2021-12-17T05:40:14Z
dc.date.available 2021-12-17T05:40:14Z
dc.date.issued 2016-04
dc.identifier.issn 0378-8741
dc.identifier.uri http://27.109.7.66:8080/xmlui/handle/123456789/772
dc.description Journal of Ethnopharmacology, 2016 en_US
dc.description.abstract Ethnopharmacological relavance: The decoction of Carica papaya Linn. leaves is used in folklore medicine in certain parts of Malaysia and Indonesia for the treatment of different types of thrombocytopenia associated with diseases and drugs. There are several scientific studies carried out on humans and animal models to confirm the efficacy of decoction of papaya leave for the treatment of disease induced and drug induced thrombocytopenia, however very little is known about the bio-active compounds responsible for the observed activity. The aim of present study was to identify the active phytochemical component of Carica papaya Linn. leaves decoction responsible for anti-thrombocytopenic activity in busulfan-induced thrombocytopenic rats. Materials and methods: Antithrombocytopenic activity was assessed on busulfan induced thrombocytopenic Wistar rats. The antithrombocytopenic activity of different bio-guided fractions was evaluated by monitoring blood platelet count. Bioactive compound carpaine was isolated and purified by chromatographic methods and confirmed by spectroscopic methods (LC-MS and 1D/2D-1H/13C NMR) and the structure was confirmed by single crystal X-ray diffraction. Quantification of carpaine was carried out by LC-MS/MS equipped with XTerra(®) MS C18 column and ESI-MS detector using 90:10 CH3CN:CH3COONH4 (6mM) under isocratic conditions and detected with multiple reaction monitoring (MRM) in positive ion mode. Results: Two different phytochemical groups were isolated from decoction of Carica papaya leaves: phenolics, and alkaloids. Out of these, only alkaloid fraction showed good biological activity. Carpaine was isolated from the alkaloid fraction and exhibited potent activity in sustaining platelet counts upto 555.50±85.17×10(9)/L with no acute toxicity. en_US
dc.language.iso en en_US
dc.publisher National Library of Medicine en_US
dc.subject Busulfan en_US
dc.subject Carica papaya Linn en_US
dc.subject Carpaine en_US
dc.subject Platelets en_US
dc.subject Thrombocytopenia. en_US
dc.title Antithrombocytopenic activity of carpaine and alkaloidal extract of carica papaya linn. Leaves in busulfan induced thrombocytopenic wistar rats en_US
dc.type Article en_US


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